SLDC means the State Load Dispatch Center as notified by the State Government.Д¬SDP means the person, if applicable, that holds in custody and administers Your Specified BEE Securities or an interest in Your Specified BEE Securities and that has been accepted in terms of section 34 of the SSA by a central securities depository as a participant in that central securities depository, which person is identified on the cover page of This BEE Contract 8 We/Us/Our means TATA AIG General Insurance Company Limited.ДPWH means the Department of Public Works and Highways of the Government of the Philippines. SDS means safety data sheet, which is a written communication regarding a hazardous chemical that meets the standards at. Mathematically, it is the sum of wasteload allocations for point sources, load allocations for non-point and natural background sources, and a margin of safety. TMDL means the total maximum daily load limitation of a parameter, representing the estimated assimilative capacity for a water body before other designated uses are adversely affected. IOSCO means the International Organisation of Securities Commissions. Pioneer or the 'Company' means Pioneer Natural Resources Company and its subsidiaries. South Caucasus/Central and South Asian (SC/CASA) state means Armenia, Azerbaijan, Georgia, Kazakhstan, Kyrgyzstan, Pakistan, Tajikistan, Turkmenistan, or Uzbekistan. Under Public Law 86-272, a state canвЂ™t impose an income tax or franchise tax based on net income on a partnership selling tangible personal property if the part- nershipвЂ™s only activity in the state is the solicitation of or- ders, which orders are approved outside the state and are filled by delivery from a point outside the state. Nexus means that a partnershipвЂ™s business activity is of such a degree that the state or foreign country has jurisdic- tion to impose an income tax or franchise tax measured by net income. вЂњCSRCвЂќ means the China Securities Regulatory Commission. Delinassios), All rights reserved.CSDCC means the China Securities Depository and Clearing Co., Ltd. Green fluorescent protein Sendai virus cell fusion cell hybrid dual-color HT-1080 heterokaryon imaging nuclear fusion red fluorescent protein.Д¬opyrightВ© 2016 International Institute of Anticancer Research (Dr. The ability to image the complex processes following cell fusion using reciprocal color-coded cancer cells will allow greater understanding of the genetic basis of malignancy. Cell fusion with heterokaryon formation and subsequent nuclear fusion resulting in hybridization may be an important natural phenomenon between cancer cells that may make them more malignant. The orange and yellow nuclei indicate nuclear fusion. Heterokaryons with red, green, orange and yellow nuclei were observed by confocal imaging, even in single hybrid cells. Multi-nucleate (heterokaryotic) cancer cells, in addition to hybrid cancer cells with single-or multiple-fused nuclei, including fused mitotic nuclei, were observed among the fused cells.

The fused cells were cultured on plastic and observed using an Olympus FV1000 confocal microscope.

The two reciprocal color-coded sublines of HT-1080 cells were fused using the Sendai virus. In order to be able to visualize these steps, we have established cancer-cell sublines from the human HT-1080 fibrosarcoma, one expressing green fluorescent protein (GFP) linked to histone H2B in the nucleus and a red fluorescent protein (RFP) in the cytoplasm and the other subline expressing RFP in the nucleus (mCherry) linked to histone H2B and GFP in the cytoplasm. However, the steps involved after initial fusion between cells, such as heterokaryon formation and nuclear fusion, have been difficult to observe in real time. Fusion of cancer cells has been studied for over half a century.

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